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中华胃肠内镜电子杂志 ›› 2025, Vol. 12 ›› Issue (02) : 126 -129. doi: 10.3877/cma.j.issn.2095-7157.2025.02.011

综述

基于结肠镜标本的结肠癌类器官构建技术及临床科研应用前景探究
张建秀1,2, 聂娇2, 杜超2,3,()   
  1. 1. 261053 潍坊,山东第二医科大学临床医学院
    2. 276000 临沂,山东第二医科大学附属临沂市人民医院消化内科
    3. 226429 威海,山东大学附属威海市立医院消化内科
  • 收稿日期:2025-03-06 出版日期:2025-05-15
  • 通信作者: 杜超
  • 基金资助:
    国家自然科学基金(81500425)山东省自然科学基金项目(ZR2021MH183)

Colorectal cancer organoids from colonoscopy biopsies: culture optimization clinical research applications

Jianxiu Zhang1,2, Jiao Nie2, Chao Du2,3,()   

  1. 1. School of Clinical Medicine,Shandong Second Medical University,Weifang 261053,China
    2. Affiliated Hospital of Shandong Second Medical University,Linyi People′s Hospital,Linyi 276000, China
    3. Affiliated Hospital of Shandong University,Weihai Municipal Hospital,Weihai 226429, China
  • Received:2025-03-06 Published:2025-05-15
  • Corresponding author: Chao Du
引用本文:

张建秀, 聂娇, 杜超. 基于结肠镜标本的结肠癌类器官构建技术及临床科研应用前景探究[J/OL]. 中华胃肠内镜电子杂志, 2025, 12(02): 126-129.

Jianxiu Zhang, Jiao Nie, Chao Du. Colorectal cancer organoids from colonoscopy biopsies: culture optimization clinical research applications[J/OL]. Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition), 2025, 12(02): 126-129.

结直肠癌(CRC)是全球第三大常见恶性肿瘤,也是癌症相关死亡的主要原因之一。 近年来,随着精准医学的发展,基于类器官模型的CRC 个体化治疗,尤其在化疗、靶向治疗及免疫治疗的疗效预测与机制探索方面,结肠镜活检已成为CRC 诊断和监测的常规手段,其获取的肿瘤组织为类器官构建提供了重要的标本来源。 利用结肠镜标本建立CRC 类器官模型,不仅能够保留患者的遗传和表型特征,还可用于药物敏感性测试、生物标志物筛选及耐药机制研究。 本文通过对结肠镜活检来源的组织进行CRC 类器官构建技术的标准化流程、技术特点及价值以及最新进展进行综述,以期为CRC 的精准化和个体化治疗策略提供参考。

Colorectal cancer (CRC) ranks as the third most prevalent malignant tumor globally and remains a leading cause of cancer-associated mortality.Recent advances in precision medicine have opened new avenues for personalized CRC treatment,with patient-derived organoid models emerging as a transformative tool in this endeavor.Colonoscopic biopsy,now a standard diagnostic and monitoring tool for CRC,provides essential tumor tissue samples for organoid construction. These specimens are particularly valuable for predicting treatment efficacy and investigating the mechanisms of chemotherapy,targeted therapy,and immunotherapy.By establishing CRC organoid models from colonoscopic biopsies,researchers can preserve patients′ genetic and phenotypic characteristics while enabling drug sensitivity testing,biomarker screening,and investigations into drug resistance mechanisms. This review summarizes standardized protocols,key technical advantages,and recent advances in CRC organoid construction using colonoscopy-derived tissues,aiming to support the development of precise and individualized therapeutic strategies for CRC.

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