A sufficient animal model of esophageal stricture after ESD

doi:10.3877/cma.j.issn.2095-7157.2025.01.008

Abstract

Abstract:

Objective

To explore and construct a sufficient animal model of esophageal stenosis associated with endoscopic submucosal dissection （ESD）.

Methods

Bama mini pigs weighing 25-30 kg（n=6） were used in this study.Each porcine was made a wound with a length of 8 cm and circumference of 5/6 on the esophageal through endoscopy.Endoscopic examination was performed on postoperative days 7，14，and 21 to evaluate wound healing and scar formation.On the 21st day，the animals were sacrificed and esophageal tissues were taken to measure and calculate the mucosal stricture rate.Performed pathological examination to evaluate the formation of scar tissue.

Results

All experimental animals had successfully performed esophageal stenosis associated with ESD，and there had been no serious complications such as massive bleeding and perforation.The esophageal mucosal stricture rate was （63.2±2.9）%，which was satisfactory.Pathological results showed that scar tissue hyperplasia occurred on the wound，leading to esophageal stenosis，which is consistent with the process and mechanism of human.

Conclusion

A wound with a length of 8 cm and a circumference of 5/6 on the esophagus of the Bama mini pig through endoscopy is a stable animal model for the formation of esophageal stenosis，which can be applied to the study of the mechanism of esophageal stenosis after ESD surgery and the exploration of related prevention and treatment methods.Its application potential still needs further research.

Key words: Esophageal stenosis, Endoscopic submucosal dissection, Animal model, Bama mini pig

Zhenyu Liu, Fei Gao, Wengang Zhan, Haiyang Li, Jiancong Feng, Ningli Chai, Enqiang Linghu. A sufficient animal model of esophageal stricture after ESD[J]. Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition), 2025, 12(01): 35-40.

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URL: https://zhwcnjdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-7157.2025.01.008

https://zhwcnjdzzz.cma-cmc.com.cn/EN/Y2025/V12/I01/35

Figures/Tables 7

特点	结果
月龄（月， $\overset{ˉ}{x}$ ±s）	12. 0±1. 0
体重（月， $\overset{ˉ}{x}$ ±s）	26. 6±1. 8
创面上缘距门齿距离（cm）	27. 2±0. 5
创面下缘距门齿距离（cm）	34. 9±0. 8
模型构建成功	6
不良反应发生
穿孔
有	0
无	6
肌层损伤
有	1
无	5
需要使用热凝钳的出血
有	2
无	4

特点	结果
月龄（月， $\overset{ˉ}{x}$ ±s）	12. 0±1. 0
体重（月， $\overset{ˉ}{x}$ ±s）	26. 6±1. 8
创面上缘距门齿距离（cm）	27. 2±0. 5
创面下缘距门齿距离（cm）	34. 9±0. 8
模型构建成功	6
不良反应发生
穿孔
有	0
无	6
肌层损伤
有	1
无	5
需要使用热凝钳的出血
有	2
无	4

编号	术前体重（kg）	术后7 d体重（kg）	术后14 d体重（kg）	术后21 d体重（kg）
1	24. 8	24. 6	23. 8	21. 6
2	26. 5	25. 9	24. 5	22. 7
3	28. 8	28. 9	27. 7	25. 4
4	28. 6	28. 8	28. 5	27. 4
5	24. 6	24. 6	24. 2	23. 2
6	26. 5	26. 3	25. 1	22. 8

编号	正常黏膜宽度（口侧，cm）	正常黏膜宽度（肛侧，cm）	最狭窄处宽度（cm）	黏膜狭窄率（%）
1	3. 3	4. 0	1. 4	61. 6
2	3. 2	4. 1	1. 5	58. 9
3	3. 0	4. 0	1. 2	65. 7
4	3. 5	3. 7	1. 2	66. 7
5	3. 4	3. 8	1. 3	63. 9
6	3. 3	4. 1	1. 4	62. 2

编号	创面部位瘢痕组织厚度（mm）	创面部位表面至固有肌层厚度（mm）	创面处瘢痕比率（%）	正常黏膜纤维组织厚度（mm）	正常黏膜表面至固有肌层厚度（mm）	正常黏膜瘢痕比率（%）
1	0. 33	0. 66	50. 0	0. 16	0. 80	20. 0
2	0. 31	0. 62	50. 0	0. 17	0. 78	21. 8
3	0. 30	0. 58	51. 7	0. 16	0. 76	21. 1
4	0. 32	0. 60	53. 3	0. 18	0. 82	22. 0
5	0. 33	0. 68	48. 5	0. 22	0. 84	26. 2
6	0. 28	0. 64	43. 8	0. 15	0. 74	20. 3

References 23

［1］	Bray F，Laversanne M，Sung H，et al.Global cancer statistics 2022： GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin，2024，74（3）：229-263.
［2］	Wang GQ，Jiao GG，Chang FB，et al.Long-term results of operation for 420 patients with early squamous cell esophageal carcinoma discovered by screening［J］.Ann Thorac Surg，2004，77（5）：1740-1744.
［3］	DaSilva LL，Aguiar PN Jr，de Lima Lopes G.Immunotherapy for Advanced Esophageal Squamous Cell Carcinoma-Renewed Enthusiasm and a Lingering Challenge［J］.JAMA Oncol，2021，7（11）：1613-1614.
［4］	令狐恩强.手术发展史的新阶段-超级微创技术［J/OL］.中华胃肠内镜电子杂志，2016，3（3）：97-98.
［5］	Seewald S，Ang TL，Gotoda T，et al.Total endoscopic resection of Barrett esophagus［J］.Endoscopy，2008，40（12）：1016-1020.
［6］	Nakagawa K，Koike T，Iijima K，et al.Comparison of the longterm outcomes of endoscopic resection for superficial squamous cell carcinoma and adenocarcinoma of the esophagus in Japan［J］.Am J Gastroenterol，2014，109（3）：348-356.
［7］	令狐恩强.新时代医学的发展方向与前景［J/OL］.中华胃肠内镜电子杂志，2021，8（2）：49-50.
［8］	Pimentel-Nunes P，Libanio D，Bastiaansen BAJ，et al.Endoscopic submucosal dissection for superficial gastrointestinal lesions：European Society of Gastrointestinal Endoscopy （ ESGE ）Guideline-Update 2022［J］.Endoscopy，2022，54（6）：591-622.
［9］	Duan Y，Jia W，Liang Y，et al.Progress in the treatment and prevention of esophageal stenosis after endoscopic submucosal dissection［J］.Clin Res Hepatol Gastroenterol，2024，48（3）：102290.
［10］	崔梅花，牟方宏，刘海芹，等.猪内镜黏膜下剥离术的模拟培训研究［J］.胃肠病学和肝病学杂志，2015，24（3）：344-347.
［11］	Ma D，Yang F，Yu Q，et al.Betulin gel alleviates esophageal stricture following endoscopic submucosal dissection：an animal study［J］.Esophagus，2024，Oct 16.Epub ahead of print.
［12］	Yano H，Sasaki F，Maeda H，et al.Effect of sprayable，highly adhesive hydrophobized gelatin microparticles on esophageal stenosis after endoscopic submucosal dissection： an experimental study in a swine model［J］.Esophagus，2024，Oct 15.Epub ahead of print.
［13］	Lai H，Yip HC，Gong Y，et al.MFGE8 in exosomes derived from mesenchymal stem cells prevents esophageal stricture after endoscopic submucosal dissection in pigs［J］.J Nanobiotechnol，2024，22（1）：143-150.
［14］	Honda M，Hori Y，Nakada A，et al.Use of adipose tissue-derived stromal cells for prevention of esophageal stricture after circumferential EMR in a canine model［J］.Gastrointest Endosc，2011，73（4）：777-784.
［15］	Chen K，Liu Y，Liu X，et al.Hyaluronic acid-modified and verteporfin-loaded polylactic acid nanogels promote scarless wound healing by accelerating wound re-epithelialization and controlling scar formation［J］.J Nanobiotechnol，2023，21（1）：241-253.
［16］	Youn JK，Lee HR，Ko D，et al.Attenuation of esophageal anastomotic stricture through remote ischemic conditioning in a rat model［J］.Sci Rep，2024，14（1）：18481.
［17］	Luo YG，Zhang XW，Zhao H，et al.A Novel Rat Model to Simulate the Benign Esophageal Stricture Induced by Endoscopic Submucosal Dissection［J］.Clin Exp Gastroenterol，2024，17（3）：41-50.
［18］	Keşim DA，Aşır F，Ayaz H，et al.The Effects of Ellagic Acid on Experimental Corrosive Esophageal Burn Injury［J］.Curr Issues Mol Biol，2024，46（2）：1579-1592.
［19］	Wang Y，Su Y，Zhu Y，et al.Research on triamcinolone-loaded thermosensitive chitosan hydrogels for preventing esophageal stricture induced by endoscopic submucosal dissection［J］.Int J Biol Macromol，2024，261（Pt 1）：129679.
［20］	陈美霞，潘碧钦，韩慧梅.早期食管癌患者ESD 后并发症危险因素分析［J］.现代医药卫生，2024，40（8）：1281-1284.
［21］	张欣欣，张耀丹，伊利亚尔·买买提力，等.早期食管癌术后食管狭窄发生情况及影响因素分析［J］.实用临床医药杂志，2024，28（12）：42-45，56.
［22］	Liu BR，Liu D，Yang W，et al.Mucosal loss as a critical factor in esophageal stricture formation after mucosal resection：a pilot experiment in a porcine model［J］.Surg Endosc，2020，34（2）：551-556.
［23］	张冰，白璟，窦晓雅，等.食管癌内镜黏膜下剥离术后发生食管狭窄风险列线图构建与验证［J］.海南医学，2024，35（8）：1185-1189.

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