Colorectal cancer organoids from colonoscopy biopsies: culture optimization clinical research applications

doi:10.3877/cma.j.issn.2095-7157.2025.02.011

Abstract

Abstract:

Colorectal cancer (CRC) ranks as the third most prevalent malignant tumor globally and remains a leading cause of cancer-associated mortality.Recent advances in precision medicine have opened new avenues for personalized CRC treatment,with patient-derived organoid models emerging as a transformative tool in this endeavor.Colonoscopic biopsy,now a standard diagnostic and monitoring tool for CRC,provides essential tumor tissue samples for organoid construction. These specimens are particularly valuable for predicting treatment efficacy and investigating the mechanisms of chemotherapy,targeted therapy,and immunotherapy.By establishing CRC organoid models from colonoscopic biopsies,researchers can preserve patients′ genetic and phenotypic characteristics while enabling drug sensitivity testing,biomarker screening,and investigations into drug resistance mechanisms. This review summarizes standardized protocols,key technical advantages,and recent advances in CRC organoid construction using colonoscopy-derived tissues,aiming to support the development of precise and individualized therapeutic strategies for CRC.

Key words: Colorectal cancer, Organoids, Colonoscopy, Precision treatment

Jianxiu Zhang, Jiao Nie, Chao Du. Colorectal cancer organoids from colonoscopy biopsies: culture optimization clinical research applications[J]. Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition), 2025, 12(02): 126-129.

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