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中华胃肠内镜电子杂志 ›› 2023, Vol. 10 ›› Issue (01) : 31 -36. doi: 10.3877/cma.j.issn.2095-7157.2023.01.010

论著

3%聚桂醇消融动物囊性肿瘤模型的有效性和安全性研究
高飞1, 李惠凯1, 冯秀雪1, 杜晨1, 韩珂1, 柴宁莉1,(), 令狐恩强1,()   
  1. 1. 100853 北京,解放军总医院第一医学中心消化内科医学部
  • 收稿日期:2023-01-05 出版日期:2023-02-15
  • 通信作者: 柴宁莉, 令狐恩强
  • 基金资助:
    国家重点研发计划(2022YFC2503604)

Cystic tumor ablation with 3% lauromacrogol solution in an animal model

Fei Gao1, Huikai Li1, Xiuxue Feng1, Chen Du1, Ke Han1, Ningli Chai1,(), Enqiang Linghu1,()   

  1. 1. Senior Department of Gastroenterology, The First Medical Center of PLA General Hospital, Beijing, China
  • Received:2023-01-05 Published:2023-02-15
  • Corresponding author: Ningli Chai, Enqiang Linghu
引用本文:

高飞, 李惠凯, 冯秀雪, 杜晨, 韩珂, 柴宁莉, 令狐恩强. 3%聚桂醇消融动物囊性肿瘤模型的有效性和安全性研究[J/OL]. 中华胃肠内镜电子杂志, 2023, 10(01): 31-36.

Fei Gao, Huikai Li, Xiuxue Feng, Chen Du, Ke Han, Ningli Chai, Enqiang Linghu. Cystic tumor ablation with 3% lauromacrogol solution in an animal model[J/OL]. Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition), 2023, 10(01): 31-36.

目的

应用浓度为3%的聚桂醇溶液对建立的动物囊性肿瘤模型进行消融治疗,以评估其消融的安全性和有效性并探索该模型模拟胰腺囊性肿瘤的实用性。

方法

自体移植法在30只雌性性成熟SD大鼠腹腔内建立子宫内膜异位囊肿模型,饲养4周后成功建模的囊肿随机分为两组分别注射3%聚桂醇溶液或生理盐水,饲养4周后打开腹腔观察囊肿消融情况,取标本进行病理学检查。

结果

最终共收集了34个囊肿,形态学方面生理盐水组囊肿术前、术后均保持半球型,而3%聚桂醇溶液组囊肿术前的半球型消融后呈扁平瘢痕状;病理学方面生理盐水组标本上皮层单层柱状上皮细胞几乎无脱落,而3%聚桂醇溶液组标本上皮层单层柱状上皮细胞全部脱落,两组标本固有层均完整。消融术后囊肿无穿孔、腹腔脏器无损伤、大鼠无死亡。统计学分析两组消融有效率表明3%聚桂醇溶液具有良好的消融疗效。

结论

3%聚桂醇溶液消融大鼠子宫异位囊肿模型安全且有效,初步表明3%聚桂醇可用于EUS-LA临床治疗。该模型一定程度上可以模拟胰腺囊性肿瘤。

Objective

To evaluate the safety and efficacy of 3% lauromacrogol solution for the ablation of animal cystic tumor models, and to explore the practicability of the model for the simulation of pancreatic cystic tumor.

Methods

Endometriosis cyst models were established in the abdominal cavity of thirty female mature SD rats by autologous transplantation.After 4 weeks of feeding, the cysts successfully modeled were randomly divided into two groups, injected with 3% lauromacrogol solution or normal saline, respectively.After 4 weeks of feeding, the abdominal cavity was opened to observe the ablation of cysts, and specimens were collected for pathological examination.

Results

A total of 34 cysts were collected. In terms of morphology, cysts in the normal saline group remained hemispherical before and after ablation, while cysts in the 3% lauromacrogol solution group showed flat scar after the ablation.In terms of pathology, almost no single layer columnar epithelial cells were ablated in the normal saline group, while all the single layer columnar epithelial cells were ablated in the 3% lauromacrogol solution group, and the lamina propria was intact in both groups. Statistical analysis of the ablation efficiency of the two groups showed that 3% lauromacrogol solution had significant ablation effect.

Conclusion

The ablation of endometriosis cyst in rats with 3% lauromacrogol solution is safe and effective, preliminary indicating that 3% lauromacrogol solution can be used for endoscopic ultrasound guided lauromacrogol ablation.This model can simulate pancreatic cystic tumors to some extent.

图1 消融过程 注:A:造模成功的囊肿;B:穿刺抽液;C:注射聚桂醇溶液或生理盐水
图2 消融囊性肿瘤模型的有效性和安全性 注:A:A组囊肿呈半球形;B:B组囊肿呈扁平瘢痕;C:周边腹部脏器无明显损伤;D:腹壁囊肿移植部位可见斑片状红斑
表1 囊肿模型消融前后的一般特征
图3 A组囊肿病理切片不同放大倍数 注:A、B:低倍镜下,囊壁具有子宫壁基本组织结构,由内向外可分为子宫内膜、肌层和外膜,内外肌层间可见较大血管,部分血管充血淤血;C、D:高倍镜下,囊腔内壁为单层柱状上皮,着色较深,部分上皮细胞呈空泡化。固有层稀疏,可见少量血管分布其中,罕见子宫腺,部分固有层和肌层可见少量嗜酸性粒细胞浸润,囊肿内具有大量成纤维细胞和纤维细胞呈错乱排列
图4 B组囊肿病理切片不同放大倍数 注:A:低倍镜下,自体移植的子宫无囊腔,无子宫内膜结构,整个组织大部分为纤维结缔组织,组织中心可见管状空隙。外膜层疏松,分布较大血管,部分血管充血淤血;B、C、D:高倍镜下,囊肿内具有大量成纤维细胞和纤维细胞呈错乱排列,纤维间可见少量嗜酸性粒细胞浸润
表2 囊肿模型消融有效率[个(%)]
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